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ebv bl lines ramos  (ATCC)


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    ATCC ebv bl lines ramos
    FIGURE 2. BL cell lines retain the <t>EBV</t> latency I expression phenotype. A, Fluorescence microscopy was performed to distinguish the EBV latency I (EBNA1 only) or latency II/III (EBNA1 and LMP1 expressed). The EBV BL cell line <t>Ramos</t> (first column), the EBV BL cell line Mutu (second column), the EBV BL cell line Ous (third column), and the EBV latency III expressing BC LCL (fourth column) were stained with EBNA1 and LMP1 Abs. Costaining with DAPI showed localization of EBNA1 in the nucleus of Mutu, Ous, and BC LCL, while LMP1 staining was localized to the cell membrane only in BC LCL. Second and fourth rows show phase-contrast pictures of the stained cells as an indication of their viability. B, Intracellular FACS staining for LMP1. Only the lymphoblastoid cell line LG2 (broken dotted line) stained positive for intracellular LMP1. Ramos (thin line), Ous (thick line), and Mutu (dotted line) did not express LMP1. C, Western blot for EBNA Ags. EBNA1 could be detected with EBV VCA high control serum in an EBNA1 transfectant of the EBV HD line L428 (L428PC5III), Ous, Mutu, and LG2, but not in the L428 mother cell line (right panels). The EBNA1- staining pattern of a higher (EBNA1) and lower (EBNA1) m.w. band and the migratory behavior under our electrophoresis conditions were confirmed by staining in parallel with the EBNA1-specific Ab 1H4. EBNA2 and EBNA3s could only be detected in the LCL LG2 with EBV VCA high control serum. Due to the differences in the EBNA1 expression level of the used cell lines, less lysate was loaded for the EBNA1 transfectant. The presented data were repeated twice.
    Ebv Bl Lines Ramos, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1435 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ebv+bl+lines+ramos/Ramos/pm12133989-53-70-93
    Average 99 stars, based on 1435 article reviews
    ebv bl lines ramos - by Bioz Stars, 2026-10
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    1) Product Images from "Epstein-Barr nuclear antigen 1-specific CD4(+) Th1 cells kill Burkitt's lymphoma cells."

    Article Title: Epstein-Barr nuclear antigen 1-specific CD4(+) Th1 cells kill Burkitt's lymphoma cells.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.169.3.1593

    FIGURE 2. BL cell lines retain the EBV latency I expression phenotype. A, Fluorescence microscopy was performed to distinguish the EBV latency I (EBNA1 only) or latency II/III (EBNA1 and LMP1 expressed). The EBV BL cell line Ramos (first column), the EBV BL cell line Mutu (second column), the EBV BL cell line Ous (third column), and the EBV latency III expressing BC LCL (fourth column) were stained with EBNA1 and LMP1 Abs. Costaining with DAPI showed localization of EBNA1 in the nucleus of Mutu, Ous, and BC LCL, while LMP1 staining was localized to the cell membrane only in BC LCL. Second and fourth rows show phase-contrast pictures of the stained cells as an indication of their viability. B, Intracellular FACS staining for LMP1. Only the lymphoblastoid cell line LG2 (broken dotted line) stained positive for intracellular LMP1. Ramos (thin line), Ous (thick line), and Mutu (dotted line) did not express LMP1. C, Western blot for EBNA Ags. EBNA1 could be detected with EBV VCA high control serum in an EBNA1 transfectant of the EBV HD line L428 (L428PC5III), Ous, Mutu, and LG2, but not in the L428 mother cell line (right panels). The EBNA1- staining pattern of a higher (EBNA1) and lower (EBNA1) m.w. band and the migratory behavior under our electrophoresis conditions were confirmed by staining in parallel with the EBNA1-specific Ab 1H4. EBNA2 and EBNA3s could only be detected in the LCL LG2 with EBV VCA high control serum. Due to the differences in the EBNA1 expression level of the used cell lines, less lysate was loaded for the EBNA1 transfectant. The presented data were repeated twice.
    Figure Legend Snippet: FIGURE 2. BL cell lines retain the EBV latency I expression phenotype. A, Fluorescence microscopy was performed to distinguish the EBV latency I (EBNA1 only) or latency II/III (EBNA1 and LMP1 expressed). The EBV BL cell line Ramos (first column), the EBV BL cell line Mutu (second column), the EBV BL cell line Ous (third column), and the EBV latency III expressing BC LCL (fourth column) were stained with EBNA1 and LMP1 Abs. Costaining with DAPI showed localization of EBNA1 in the nucleus of Mutu, Ous, and BC LCL, while LMP1 staining was localized to the cell membrane only in BC LCL. Second and fourth rows show phase-contrast pictures of the stained cells as an indication of their viability. B, Intracellular FACS staining for LMP1. Only the lymphoblastoid cell line LG2 (broken dotted line) stained positive for intracellular LMP1. Ramos (thin line), Ous (thick line), and Mutu (dotted line) did not express LMP1. C, Western blot for EBNA Ags. EBNA1 could be detected with EBV VCA high control serum in an EBNA1 transfectant of the EBV HD line L428 (L428PC5III), Ous, Mutu, and LG2, but not in the L428 mother cell line (right panels). The EBNA1- staining pattern of a higher (EBNA1) and lower (EBNA1) m.w. band and the migratory behavior under our electrophoresis conditions were confirmed by staining in parallel with the EBNA1-specific Ab 1H4. EBNA2 and EBNA3s could only be detected in the LCL LG2 with EBV VCA high control serum. Due to the differences in the EBNA1 expression level of the used cell lines, less lysate was loaded for the EBNA1 transfectant. The presented data were repeated twice.

    Techniques Used: Expressing, Fluorescence, Microscopy, Staining, Membrane, Western Blot, Control, Transfection, Electrophoresis

    Related Articles

    Cell Culture:

    Article Title: Epstein-Barr nuclear antigen 1-specific CD4(+) Th1 cells kill Burkitt's lymphoma cells.
    Article Snippet: .. The EBV-transformed B cell lines LRM (HLA-A2, -B44, -DRB1*0401, -DQA1*03, -DQB1*0301, -DP4) (34), LG2 (HLA-A2, -B*27052, -Cw1, -DRB1*0101, -DQA*0101, -DQB1*0501, -DPA1*0101, -DPB1*0201) (35), BC LCL (HLA-A*3001, -A*3201, -B*1302, -B*4402, -C*0602, -C*0501, -DRB1*0401, -DRB1*0701, -DRB4*01, -DQB1*0302, -DQB1*0201), NL LCL (HLA-A1, -A3, -B7, -B8, -DR7, -DR17, -DRw52, -DRw53, -DQ2), Ag876 (HLA-DR7, -DR11; a gift from R. Khanna), the B/T hybrid T2 (HLA-A2, -B5, -Cw1, -DR , -DQ , -DP ) (36), the EBV BL lines Ramos (HLA-DRB1*0701, -DRB4*01) (37), the EBV Hodgkin’s lymphoma cell line RPMI6666 (HLA-A*0201, -A*0301, -B*0702/15, -B*1801/05, -C*0702, -C*0701/06, -DRB1*1501, -DRB5*01, -DQB*0602/11; American Type Culture Collection, Manassas, VA), and the EBV Hodgkin’s lymphoma cell line L428 (HLA-A*0301, -B*3503, -C*0401/03/05/07, -DRB1*1201/06, -DRB3*02, -DQB1*0301/09; a gift from M. Vockerodt (University of Cologne, Cologne, Germany) and D. Kube (University of Göttingen, Göttingen, Germany)) (38) were cultured in RPMI 1640 10% FCS glutamine gentamicin. ..



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    ebv bl lines ramos  (ATCC)
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    FIGURE 2. BL cell lines retain the <t>EBV</t> latency I expression phenotype. A, Fluorescence microscopy was performed to distinguish the EBV latency I (EBNA1 only) or latency II/III (EBNA1 and LMP1 expressed). The EBV BL cell line <t>Ramos</t> (first column), the EBV BL cell line Mutu (second column), the EBV BL cell line Ous (third column), and the EBV latency III expressing BC LCL (fourth column) were stained with EBNA1 and LMP1 Abs. Costaining with DAPI showed localization of EBNA1 in the nucleus of Mutu, Ous, and BC LCL, while LMP1 staining was localized to the cell membrane only in BC LCL. Second and fourth rows show phase-contrast pictures of the stained cells as an indication of their viability. B, Intracellular FACS staining for LMP1. Only the lymphoblastoid cell line LG2 (broken dotted line) stained positive for intracellular LMP1. Ramos (thin line), Ous (thick line), and Mutu (dotted line) did not express LMP1. C, Western blot for EBNA Ags. EBNA1 could be detected with EBV VCA high control serum in an EBNA1 transfectant of the EBV HD line L428 (L428PC5III), Ous, Mutu, and LG2, but not in the L428 mother cell line (right panels). The EBNA1- staining pattern of a higher (EBNA1) and lower (EBNA1) m.w. band and the migratory behavior under our electrophoresis conditions were confirmed by staining in parallel with the EBNA1-specific Ab 1H4. EBNA2 and EBNA3s could only be detected in the LCL LG2 with EBV VCA high control serum. Due to the differences in the EBNA1 expression level of the used cell lines, less lysate was loaded for the EBNA1 transfectant. The presented data were repeated twice.
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    ebv negative bl cell lines  (ATCC)
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    FIGURE 2. BL cell lines retain the <t>EBV</t> latency I expression phenotype. A, Fluorescence microscopy was performed to distinguish the EBV latency I (EBNA1 only) or latency II/III (EBNA1 and LMP1 expressed). The EBV BL cell line <t>Ramos</t> (first column), the EBV BL cell line Mutu (second column), the EBV BL cell line Ous (third column), and the EBV latency III expressing BC LCL (fourth column) were stained with EBNA1 and LMP1 Abs. Costaining with DAPI showed localization of EBNA1 in the nucleus of Mutu, Ous, and BC LCL, while LMP1 staining was localized to the cell membrane only in BC LCL. Second and fourth rows show phase-contrast pictures of the stained cells as an indication of their viability. B, Intracellular FACS staining for LMP1. Only the lymphoblastoid cell line LG2 (broken dotted line) stained positive for intracellular LMP1. Ramos (thin line), Ous (thick line), and Mutu (dotted line) did not express LMP1. C, Western blot for EBNA Ags. EBNA1 could be detected with EBV VCA high control serum in an EBNA1 transfectant of the EBV HD line L428 (L428PC5III), Ous, Mutu, and LG2, but not in the L428 mother cell line (right panels). The EBNA1- staining pattern of a higher (EBNA1) and lower (EBNA1) m.w. band and the migratory behavior under our electrophoresis conditions were confirmed by staining in parallel with the EBNA1-specific Ab 1H4. EBNA2 and EBNA3s could only be detected in the LCL LG2 with EBV VCA high control serum. Due to the differences in the EBNA1 expression level of the used cell lines, less lysate was loaded for the EBNA1 transfectant. The presented data were repeated twice.
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    FIGURE 2. BL cell lines retain the EBV latency I expression phenotype. A, Fluorescence microscopy was performed to distinguish the EBV latency I (EBNA1 only) or latency II/III (EBNA1 and LMP1 expressed). The EBV BL cell line Ramos (first column), the EBV BL cell line Mutu (second column), the EBV BL cell line Ous (third column), and the EBV latency III expressing BC LCL (fourth column) were stained with EBNA1 and LMP1 Abs. Costaining with DAPI showed localization of EBNA1 in the nucleus of Mutu, Ous, and BC LCL, while LMP1 staining was localized to the cell membrane only in BC LCL. Second and fourth rows show phase-contrast pictures of the stained cells as an indication of their viability. B, Intracellular FACS staining for LMP1. Only the lymphoblastoid cell line LG2 (broken dotted line) stained positive for intracellular LMP1. Ramos (thin line), Ous (thick line), and Mutu (dotted line) did not express LMP1. C, Western blot for EBNA Ags. EBNA1 could be detected with EBV VCA high control serum in an EBNA1 transfectant of the EBV HD line L428 (L428PC5III), Ous, Mutu, and LG2, but not in the L428 mother cell line (right panels). The EBNA1- staining pattern of a higher (EBNA1) and lower (EBNA1) m.w. band and the migratory behavior under our electrophoresis conditions were confirmed by staining in parallel with the EBNA1-specific Ab 1H4. EBNA2 and EBNA3s could only be detected in the LCL LG2 with EBV VCA high control serum. Due to the differences in the EBNA1 expression level of the used cell lines, less lysate was loaded for the EBNA1 transfectant. The presented data were repeated twice.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Epstein-Barr nuclear antigen 1-specific CD4(+) Th1 cells kill Burkitt's lymphoma cells.

    doi: 10.4049/jimmunol.169.3.1593

    Figure Lengend Snippet: FIGURE 2. BL cell lines retain the EBV latency I expression phenotype. A, Fluorescence microscopy was performed to distinguish the EBV latency I (EBNA1 only) or latency II/III (EBNA1 and LMP1 expressed). The EBV BL cell line Ramos (first column), the EBV BL cell line Mutu (second column), the EBV BL cell line Ous (third column), and the EBV latency III expressing BC LCL (fourth column) were stained with EBNA1 and LMP1 Abs. Costaining with DAPI showed localization of EBNA1 in the nucleus of Mutu, Ous, and BC LCL, while LMP1 staining was localized to the cell membrane only in BC LCL. Second and fourth rows show phase-contrast pictures of the stained cells as an indication of their viability. B, Intracellular FACS staining for LMP1. Only the lymphoblastoid cell line LG2 (broken dotted line) stained positive for intracellular LMP1. Ramos (thin line), Ous (thick line), and Mutu (dotted line) did not express LMP1. C, Western blot for EBNA Ags. EBNA1 could be detected with EBV VCA high control serum in an EBNA1 transfectant of the EBV HD line L428 (L428PC5III), Ous, Mutu, and LG2, but not in the L428 mother cell line (right panels). The EBNA1- staining pattern of a higher (EBNA1) and lower (EBNA1) m.w. band and the migratory behavior under our electrophoresis conditions were confirmed by staining in parallel with the EBNA1-specific Ab 1H4. EBNA2 and EBNA3s could only be detected in the LCL LG2 with EBV VCA high control serum. Due to the differences in the EBNA1 expression level of the used cell lines, less lysate was loaded for the EBNA1 transfectant. The presented data were repeated twice.

    Article Snippet: The EBV-transformed B cell lines LRM (HLA-A2, -B44, -DRB1*0401, -DQA1*03, -DQB1*0301, -DP4) (34), LG2 (HLA-A2, -B*27052, -Cw1, -DRB1*0101, -DQA*0101, -DQB1*0501, -DPA1*0101, -DPB1*0201) (35), BC LCL (HLA-A*3001, -A*3201, -B*1302, -B*4402, -C*0602, -C*0501, -DRB1*0401, -DRB1*0701, -DRB4*01, -DQB1*0302, -DQB1*0201), NL LCL (HLA-A1, -A3, -B7, -B8, -DR7, -DR17, -DRw52, -DRw53, -DQ2), Ag876 (HLA-DR7, -DR11; a gift from R. Khanna), the B/T hybrid T2 (HLA-A2, -B5, -Cw1, -DR , -DQ , -DP ) (36), the EBV BL lines Ramos (HLA-DRB1*0701, -DRB4*01) (37), the EBV Hodgkin’s lymphoma cell line RPMI6666 (HLA-A*0201, -A*0301, -B*0702/15, -B*1801/05, -C*0702, -C*0701/06, -DRB1*1501, -DRB5*01, -DQB*0602/11; American Type Culture Collection, Manassas, VA), and the EBV Hodgkin’s lymphoma cell line L428 (HLA-A*0301, -B*3503, -C*0401/03/05/07, -DRB1*1201/06, -DRB3*02, -DQB1*0301/09; a gift from M. Vockerodt (University of Cologne, Cologne, Germany) and D. Kube (University of Göttingen, Göttingen, Germany)) (38) were cultured in RPMI 1640 10% FCS glutamine gentamicin.

    Techniques: Expressing, Fluorescence, Microscopy, Staining, Membrane, Western Blot, Control, Transfection, Electrophoresis